The aim of this study was to detect possible chromosomal aberrations in subjects occupationally exposed to ionizing radiation. The results of five-year analyses of chromosomal status of 852 subjects occupationally exposed to ionizing radiation are presented in this study. In the period 1996-2000 rise of overall incidence of subjects with chromosomal aberrations was (9.0-24.1%). Special attention was focused to three groups of subjects radiologists, x-ray technicians and professionals in nuclear medicine. Overt this period professionals in nuclear medicine and x-ray technicians showed the rise, while radiologists showed a fall in the incidence of chromosomal aberrations. High values of the incidence of subjects with chromosomal aberrations were recorded in professionals in nuclear medicine result from external radiation and internal contamination. Special care should be focused at improvement of working conditions and highlight the importance of adequate occupational protection measures. Besides, health check ups of professionals occupationally exposed to ionizing radiation and their systematic educations should be improved.