Dual-energy X-ray absorptiometry (DXA) provides accurate and precise body composition and adiposity assessment, and enables development and calibration of the anthropometric equations. Seidel's and Lean's equation as well as DXA are used to determine total body fat mass percentage (FM %). The aim of this study was to evaluate the accuracy of Seidel's and Lean's equation in comparison to DXA FM% estimate. DXA was performed in 88 women with mean body mass index (BMI) (28.22±5.12 kg/m2) and age (50.79±13.57yr.), divided in 4 groups according to their BMI: 1st gr.<25 kg/m2; 2nd gr. 25-29,9 kg/m2; 3rd gr. 30-34,9 kg/m2; and 4th gr. 35-40 kg/m2. Seidel's equation (FM% = 4,201/D - 3,813 x 100; D= 1,1369 - 0,0598 x logSUMA; SUMA= triceps skinfold (SF) + biceps SF + subscapular SF + suprailiac SF) and Lean's equation (FM% = 0,439 x waist circumference + 0.221 x age - 9,4) calculated FM%, and it was compared to FM% determined by DXA. Total FM% determined by Lean's equation (42.67±7.14%) was not significantly different compared to FM% determined by DXA (41.44±7.14%), but FM% determined by Seidel's method was significantly lower (28.06±3.46%). FM% determined by Lean's equation was not significantly different, but FM% determined by Seidel's equation was lower compared to DXA in all groups divided according to their BMI. FM% determined by Lean's equation correlated significantly with FM% determined by DXA (p<0.0001). DXA examination discovered that Seidel's equation underestimated FM% in all groups, and it was confirmed that Lean's equation provide accurate and precise FM% assessment, and it could be used in clinical practice.