The aim of research was to determine the shape and frequency of median diastema in orthodontic patients of the Dental Clinic in Niš. The research was carried out on 538 (225 males and 313 females) study models. Three shapes of median diastema are defined (parallel, divergent and convergent) and the diameler in the gingival and incisal region was measured by digital nonius. Median diastema is present in 65 patients(12,09%), 31 patients of which are female (9,90%) and 34 patients are male (15, 11%). According to shape, a parallel median diastema is the most frequent in patients to both sexes (76,47% in males and 67,74% and 67,74% in females).