Eight anthropometric measures and six motor ability tests were performed on the sample of 407 girls from schools in Novi Sad and 317 girls from schools in Split (Croatia), aged 11-14. It was determined that in the entire system of variables there were statistically significant differences between girls from Novi Sad and Split in all four analyzed age categories. Results showed that girls from Split had significantly higher values of height and weight, chest circumference and upper arm and forearm volumes in the older analyzed age groups. Subcutaneous adipose tissue was significantly expressed in Novi Sad's girls in the 13th and 14th years of age. Coordination of the whole body, the frequency of alternative arm movement and flexibility were significantly better in girls from Split aged 12, 13 and 14. Girls from Novi Sad were significantly better in the running speed only at 11 years of age, with better flexibility in the 12th year, and the showed better staying power of arms and shoulders in all analyzed age categories. The least differences were shown in the 11 years of age, and the largest occurred in the 13th and 14th year, in favor of a girls from Split. This may indicate that the girls from Split come earlier in puberty period, and mature earlier.